11 June 2009

Introduction to the Male Half of Reproductive Biology



Introduction to the Male Half of Reproductive Biology
By:Genevieve Griffiths
University Of Delaware

Why Study Sperm Biology?
* One in six couples are infertile.
* In 40 per cent of cases the problem lies exclusively with the male, known as Male Factor Infertility.
* One in 25 males have a low sperm count, and one in 35 are sterile.
* With appropriate treatment, many couples struggling with male factor infertility are able to conceive.

Sexual Reproduction
* Occurs when two gametes (sperm + egg, 1N or ½ genome) combine genetic material (DNA) to form a zygote (embryo, 2N or 1 genome)
* Recombination permits genetic flexibility within a population (can lead to evolution)
* Offspring have characteristics from both parents as well as those unique from parents
* Sperm production is known as spermatogenesis
* Five mitotic divisions produce 16 primary spermatocytes from a single cell
* Two meiotic divisions produce 64 spermatids

Spermatogenesis
* Mitosis (2N (46 chromosomes) to 2N) must occur to create many cells from a single cell
* Meiosis (2N to 1N (23 chromosomes)) must occur to divide DNA in half

After meiosis, sperm dramatically change shape
Testes and Epididymis
* Spermatogeneis occurs in the seminiferous tubules of the testes
* Sperm maturation occurs in the epididymis

Immature Sperm
Mature Sperm
Mammalian Fertilization
cumulus
cells
zona
pellucida
Egg
Mammalian Fertilization
cumulus
cells
Oocyte

Introduction to the Male Half of Reproductive Biology.ppt

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Male Female Reproductive System



Male Female Reproductive System

Male Reproduction -Testis
* Compartments
Testicular cell types
* Germ cells - spermatogenesis
Molecular Structure of LH and FSH
Spermatogonium
Resting cell and 3 active cells
Mitotic divisions
Type B spermatogonia
1o spermatocytes
Meiosis I
2o spermatocytes
spermatids
Mitotic divisions
Meiosis II
Spermatogenesis
spermatozoa
Sperm Maturation vs Capacitation
Sperm vs Seminal Plasma vs Semen
Somatic cells
Sertoli cell function
Major Actions of Testosterone
Androgens
Actions of androgens and metabolites
* Estrogens
Relative abundance of steroids in males and females
Major Components of the Reproductive Female System
GnRH pulse generator
Progesterone Effects on the Pulse Generator
Effects of Estradiol on the Pulse Generator
Ovarian Cell Types
Germ Cells
Stages of Follicular development
Ovulation
Corpus Luteum formation
Ovarian Cycle
Human Menstrual Cycle
Maternal Recognition of Pregnancy
Physiological effects of ovarian steroids
* Estrogens
* Progesterone
Sexual Differentiation
Duct Development
MIH
testosterone
External Genitalia
Response to dihydrotestosterone
Brain
Sexual Differentiation of the Hypothalamus

Male Female Reproductive System.ppt

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REPRODUCTIVE SYSTEM



REPRODUCTIVE SYSTEM

REPRODUCTIVE SYSTEM: Design
* Not for Homeostasis; Instead to Perpetuate the Species
* Sexual Reproduction Results in Genetic Variability
* Internal Fertilization & Gestation
* One Offspring per Pregnancy is Typical

REPRODUCTIVE SYSTEM: Functions
* Production & Support of Gametes
* Formation, Transport & Delivery of Sperm
* Formation & Transport of Ova
* Protect & Support Developing Embryo, and Nourish Fetus
* Deliver the Fetus

REPRODUCTIVE SYSTEM: Overview of Anatomy
* Reproductive Organs
* Associated Ducts
* Accessory Glands
* External Genitalia

MALE ANATOMY: TESTES
* From the Greek for “witness” (e.g., testify)
* Essential organs of reproduction in the male (Male Gonad)
* Site of sperm production
* Suspended in scrotum by spermatic cord
* Oval, ~4.5 cm long, 10.5 - 14 gms

MALE ANATOMY: SCROTUM
* Pouch of skin and fascia evaginated from anterior abdominal wall
* Subdivided into two lateral compartments, indicated by Raphe (Ridge)
* Houses testes, keeps them cool (93F)
* Cremaster muscle brings testes closer to body
* Dartos muscle causes wrinkling

MALE ANATOMY: TESTES
* Develop initially in abdominal cavity (retroperitoneally)
* Descend into scrotum
* Seminiferous tubules: Sites of sperm production
* Interstitial Cells: Secrete Testosterone
* Efferent ductules: Carry sperm from testes
* Epididymis:

MALE ANATOMY: SPERMATIC CORD
* Contains structures passing to and from testes
* Coverings derived from abdominal wall
* Contents include:
o Vas deferens
o Spermatic artery and vein
o Spermatic nerve
o Lymph vessel

MALE ANATOMY: VAS (DUCTUS) DEFERENS
* Carries sperm from epididymis to seminal vesicle
* Passes through inguinal canal into body cavity
* Crosses surface of urinary bladder
* Joins with duct of seminal vesicle to form the ejaculatory duct
* Vasectomy

MALE ACCESSORY GLANDS: SEMINAL VESICLES
* Paired structures posterior to urinary bladder
* Secrete ~60% of seminal fluid
* Fluid is sugary, alkaline
* Rich in carbohydrates, Vitamin C

MALE ACCESSORY GLANDS : PROSTATE GLAND
* Located inferior to urinary bladder, anterior to rectum
* Surrounds prostatic urethra
* Secretes ~25% of seminal fluid
* Enzymes to Liquify Semen

MALE ACCESSORY GLANDS : BULBOURETHRAL GLANDS
* Also called Cowper’s glands
* Lateral to membranous urethra
* Secrete a drop of alkaline mucus
o Cleans, lubricates urethra

MALE ANATOMY: PENIS

* Male copulatory organ
* Functions to introduce sperm into female
* Consists of three erectile bodies
o Two corpora cavernosa
o One corpus spongiosum

MALE ANATOMY: PENIS
* Corpus spongiosum
* Corpora cavernosa
* Prepuce (foreskin):

MALE PHYSIOLOGY: SPERMATOGENESIS
o Occurs in seminiferous tubules
o Spermatogonia divide mitotically
o One Spermatocyte eventually gives rise to Four Sperm via meiosis
o Y Sperm are Smaller, Faster, Prefer Alkaline Environment
o X Sperm are Larger, Stronger, Prefer Acidic Environment


MALE SEXUAL RESPONSE
* Erection
o Arteries dilate, increasing blood in spongy tissue
o Constricts veins, causes erection
* Ejaculation (expulsion)
o Semen passes through urethra
o Contractions of urethra & penile musculature


MALE HORMONAL CONTROLS
* Gonadotropic Hormones
* Testosterone (Male Sex Hormone)

FEMALE ANATOMY: OVARY
* Female Gonad
* All ova arrested at prophase I at birth
* Paired, oval, almond sized
* Supported by ligaments

FEMALE ANATOMY: UTERINE (Fallopian) TUBES
* Site of Fertilization
* Paired, ~10 cm long
* Attach to uterus, one on each side
* Transport via Cilia & Peristalsis

FEMALE ANATOMY: UTERUS
* Usually unpaired; hollow, muscular, pear-shaped organ
* Receives uterine tubes, empties into vagina
* Site of Implantation & Development
* Supported by ligaments
* Uterine wall has three layers

FEMALE ANATOMY: UTERUS
* Fundus: Arches above entry of uterine tubes
* Body: Large triangular lumen
* Isthmus: Narrowed region
* Cervix:

FEMALE ANATOMY: VAGINA
* Functions:
* Located between urethra and rectum
* Lined with stratified squamous E.T.
* Acidic environment (Resident Bacteria)
* Fornix: Circular recess around cervix; upper portion of vagina
* Hymen:
* Bartholin’s glands:

FEMALE ANATOMY: VULVA (External Genitalia)
* Mons pubis: Fatty mound
* Labia majora: Outer Folds of skin, Homologous to scrotum
* Labia minora: Inner folds, Encircle clitoris, forming prepuce
* Clitoris: Homologous to penis, corpora cavernosa

FEMALE ANATOMY: CLITORIS
* Foreskin or Prepuce
* Function – Sexual Pleasure
* Orgasm controlled by Sympathetic Division of ANS

FEMALE ANATOMY: BREASTS
* Structurally – Integument
* Functionally – Reproductive

HORMONAL CONTROL: ADULT FEMALE
* Four hormones involved:

THE OVARIAN CYCLE
* Includes:

FOLLICLE DEVELOPMENT
* Up to 20 follicles begin maturation process monthly
* Only one secondary follicle reaches maturity
* Others undergo atresia (degradation)
* Controlled primarily by FSH
* Causes follicle cells to secrete estrogen


OVULATION
* Caused by LH surge
* Day 14 (28-day cycle)
* Expels ovum into abdominal cavity
* Fimbriae on uterine tube sway vigorously, produce a current
* Fimbriae scratch Graafian follicle, rupture it, pull ovum into tube

FORMATION OF CORPUS LUTEUM
* Ruptured Graafian follicle becomes corpus luteum
* Corpus luteum secretes estrogen, progesterone
* Maintains endometrial lining during pregnancy

THE UTERINE CYCLE

* Proliferative phase
o Follows Menstruation
o Estrogen from follicle stimulates proliferation of endometrium
* Secretory phase
o Follows Ovulation
o Progesterone from corpus luteum stimulates secretion by endometrium
* Menstrual phase

REPRODUCTIVE SYSTEM.ppt

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