Molecular techniques used in medical diagnosis

Molecular techniques used in medical diagnosis

Separation of DNA Fragments by Gel Electrophoresis
Restriction Endonucleases

Methods of Sequence Analysis
The human genome project
Big Dye Sequencing
Polymerase Chain Reaction
PCR Analysis - Multiple rounds of amplification
Polymorphisms
Types of polymorphisms
X-linked Inheritance
RFLP Analysis
Southern Blotting
An example of VNTR linkage analysis
The use of PCR in Forensics Medicine
Fragile X syndrome is caused by the expansion of a VNTR region
DNA microarray analysis
Preparation of cDNA from mRNA

Molecular techniques used in medical diagnosis.ppt